Enzyme Lab

Procedure:

1.       Line up three dry test tubes and label them 1, 2 and 3.

2.       Using a 10.00ml graduated cylinder and pipette, add 3.00ml of hydrogen peroxide into first test tube. This test tube will be used as the standard.

3.       3.00ml of hydrogen peroxide and 3.00ml of water are added into the same 10.00ml cylinder. Pour the solution into a clean, dry test tube to mix it. Using the same 10.00ml graduated cylinder and pipette measure 3.00ml of this solution, and pour it into test tube2.

4.       3.00ml of hydrogen peroxide and 6.00ml of water are added into the same 10.00ml cylinder. Pour the solution into a clean, dry test tube to mix it. Using the same 10.00ml graduated cylinder and pipette measure 3.00ml of this solution, and pour it into test tube3.

5.       Overflow pan is filled with water.

6.       Add water to fill the 1L graduated cylinder to the top and invert it into the pan. Record the volume of gas in the cylinder.

7.       Put 5 disks of liver in a 20.00ml Erlenmeyer flask. A rubber stopper with a funnel and tubing that extends into the 1L cylinder.

8.       Put the rubber stopper on the flask and pour the first test tube’s solution into the funnel. Cap the funnel with finger immediately and shake the flask.

9.       Record the time as long as the gas produced.

10.   Record the new volume of gas in the cylinder.

11.   Repeat step 6 -10 for test tube2 and test tube3.

12.   Clean up

Result:

	Test tube1	Test tube2	Test tube3
Volume of H2O2	3.00ml	3.00ml	3.00ml
Volume of H2O	0.00ml	3.00ml	6.00ml
Volume of the solution add into test-tube	3.00ml	3.00ml	3.00ml
Original volume of gas in the cylinder	75.00ml	66.00ml	67.00ml
Amount of gas after reaction in the cylinder	59.00ml	55.00ml	63.00ml
Total amount of gas produced	16.00ml	11.00ml	5.00ml
The time it used	11.60seconds	15.31seconds	13.52seconds